By M.J. Berridge, J.E. Treherne, V.B. Wigglesworth (Eds.)
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Additional resources for Advances in Insect Physiology, Vol. 15
In addition, when material is treated with fixatives including calcium, subsequent treatment with uranyl acetate in the presence of calcium leads to images comparable to lanthanum-treated ones, as the uranium fills the extracellular space, delineating the structures that lie therein. , 1975), in contrast to the more conventional “uranyl acetate en bloc staining” (Farquhar and Palade, 1965) for enhancing membrane structure as referred to earlier. Depending on the plane of section, the stained intercellular cleft will reveal the non-opaque intercellular structures either along the longitudinal axis or transverse axis, while tangential sections elucidate the threedimensional structure or interrelationships of these junctional components.
1975), in contrast to the more conventional “uranyl acetate en bloc staining” (Farquhar and Palade, 1965) for enhancing membrane structure as referred to earlier. Depending on the plane of section, the stained intercellular cleft will reveal the non-opaque intercellular structures either along the longitudinal axis or transverse axis, while tangential sections elucidate the threedimensional structure or interrelationships of these junctional components. Such methods often clarify the detailed features of the junction under consideration because exclusion of tracer by a structure usually gives a clearer impression of its characteristics than positive staining, which cannot distinguish structure from background as easily or effectively.
SKAER lucent. The intercellul:ir s p i c e in the ;ire;i\ ot the junction. in transversc section. is \ l i s h t l y larger ( c t r . I 7 n m ) than in convcnticnally \t;iinecl preparations d u e t o optical f u \ i o n o f the electrov-dense intercellular pace with the osmiophilic outer nienihrane leiitlets on either aide o f it. I he w p t a show up a s electron-lucent b a r s often nar-I-owcr- (4-5 n m ; Satir a n d (iilula. 1973) than i n nci n - in ti It r;i t ecl \ peci ni e n \ . In t;ingenti;il section\ t n c septa are \trikinglj reve;iled ;I\ clear undulating rihtmns set in ; i n electron-de:iw lake ot \tain (Fig.
Advances in Insect Physiology, Vol. 15 by M.J. Berridge, J.E. Treherne, V.B. Wigglesworth (Eds.)