By Frank J. Dixon (Ed.)
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Extra resources for Advances in Immunology, Vol. 42
En five or more viruses were used in the panel, the number of RP was estimated by the method of Briles and Carroll (1981). Closed circles represent the number of anti-HA RP for primary adult spleen; open circles are values for sIg- bone marrow cells. The broken line indicates the maximum number of RP definable with a given number of heterologous viruses. , 1983). 40 NORMAN R. , 1983; Klinman and Stone, 1983). However, the term “predominant clonotype” applies to a variety of circumstances wherein immunized animals of a given murine strain ultimately produce an antibody population that contains a high representation of a single clonotype or clonotype family.
In addition, it appears that at the time that PC-responsive cells are first appearing in their spleen (days 5-8), neonatal mice are most susceptible to anti-T15 suppression. , 1977).
Antiidiotypic assays for T15 as well as cDNA probes for the vh S107 and various other vh gene families were used to assess V, gene segment utilization in these hybridomas. It was found that all tested PC-specific hybridoma antibodies obtained from bone marrow cells of young mice utilized the S107 V, gene segment. Although both T15' and some T15antibodies obtained from fusions of bone marrow cells of aged mice also used S107 V, gene segments, the majority of PC-specific hybridoma antibodies derived from bone marrow cells of aged mice were T15- and utilized V, gene segments of families other than S107.
Advances in Immunology, Vol. 42 by Frank J. Dixon (Ed.)